7AAQ

sugar/H+ symporter STP10 in outward occluded conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-20
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97942
Spacegroup name	P 21 21 21
Unit cell lengths	59.496, 92.780, 119.788
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.390 - 1.810
R-factor	0.1887
Rwork	0.187
R-free	0.21200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6h7d
Data reduction software	DIALS (1-14-4)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	59.900	59.930	1.840
High resolution limit [Å]	1.810	4.910	1.810
Rmerge	0.168	0.047	2.501
Rmeas	0.176	0.050	2.490
Rpim	0.053	0.015	0.788
Total number of observations	665695	7411	47580
Number of reflections	61268	3317	2964
<I/σ(I)>	7.7	25.2	1.1
Completeness [%]	100.0	100	98.7
Redundancy	11	22.2	1
CC(1/2)	0.998	0.999	0.651

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	4	292.15	0.1-0.15M Ammonium Acetate, 0.1M Sodium Citrate and 36-40% PEG400