7A9Z
Structural comparison of cellular retinoic acid binding protein I and II in the presence and absence of natural and synthetic ligands
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 120 |
Detector technology | PIXEL |
Collection date | 2020-01-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9763 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 127.545, 127.545, 52.161 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 110.457 - 2.410 |
Rwork | 0.205 |
R-free | 0.25000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1cbr |
RMSD bond length | 0.009 |
RMSD bond angle | 1.559 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0266) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 110.460 | 2.500 |
High resolution limit [Å] | 2.410 | 2.410 |
Rmerge | 0.107 | 6.347 |
Rmeas | 0.392 | 6.751 |
Rpim | 0.123 | 2.287 |
Number of reflections | 19102 | 33328 |
<I/σ(I)> | 7.1 | |
Completeness [%] | 99.9 | |
Redundancy | 19.2 | 16.8 |
CC(1/2) | 0.994 | 0.398 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | PEG mixture |