7A99
Crystal structure of the Phe57Trp mutant of the arginine-bound form of domain 1 from TmArgBP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-05-11 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.54 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 37.480, 67.217, 102.411 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 56.190 - 1.790 |
R-factor | 0.1807 |
Rwork | 0.178 |
R-free | 0.23270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6gpc |
RMSD bond length | 0.018 |
RMSD bond angle | 1.841 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 56.190 | 1.870 |
High resolution limit [Å] | 1.790 | 1.790 |
Rpim | 0.033 | 0.317 |
Number of reflections | 25021 | 3013 |
<I/σ(I)> | 27.4 | |
Completeness [%] | 99.1 | |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Sodium acetate trihydrate pH 4.5, 25% w/v Polyethylene glycol 3,350 |