7A8X

Complex of rice blast (Magnaporthe oryzae) effector protein AVR-PikC with the HMA domain of Pikh-1 from rice (Oryza sativa)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-18
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	65.351, 83.121, 107.842
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.922 - 2.300
Rwork	0.210
R-free	0.25590
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7a8w
RMSD bond length	0.008
RMSD bond angle	1.488
Data reduction software	Aimless (0.7.4)
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.8.3)
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	107.840	107.840	2.380
High resolution limit [Å]	2.300	8.910	2.300
Rmerge	0.061	0.018	0.927
Rmeas	0.064	0.019	0.968
Rpim	0.018	0.006	0.278
Number of reflections	25847	543	2570
<I/σ(I)>	22.7	87.1	2.5
Completeness [%]	96.4	99.6	100
Redundancy	13.1	10.7	12
CC(1/2)	1.000	1.000	0.864

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.12 M Monosaccharides (0.2M D-Glucose; 0.2M D-Mannose; 0.2M D-Galactose; 0.2M L-Fucose; 0.2M D-Xylose; 0.2M N-Acetyl-D-Glucosamine); 0.1 M Buffer system 1 (1 M Imidazole; MES monohydrate (acid)) pH 6.5; 50% v/v Precipitant mix 1 (40% v/v PEG 500; MME; 20 % w/v PEG 20000)