7A8V
Crystal structure of Polysaccharide monooxygenase from P.verruculosum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2020-04-16 |
| Detector | Bruker Platinum 135 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 63 |
| Unit cell lengths | 132.145, 132.145, 39.952 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.036 - 1.950 |
| R-factor | 0.161874169645 |
| Rwork | 0.161 |
| R-free | 0.18669 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4eis |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.013 |
| Data reduction software | PROTEUM PLUS |
| Data scaling software | PROTEUM PLUS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.040 | 2.050 |
| High resolution limit [Å] | 1.940 | 1.950 |
| Rmerge | 0.104 | 0.284 |
| Number of reflections | 29254 | 3860 |
| <I/σ(I)> | 10.28 | |
| Completeness [%] | 99.0 | |
| Redundancy | 4.04 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | cobalt chloride, MES, ammonium sulfate |
