7A8T

Crystal structure of sarcomeric protein FATZ-1 (mini-FATZ-1 construct) in complex with rod domain of alpha-actinin-2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-03
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.9762
Spacegroup name	P 31 2 1
Unit cell lengths	103.440, 103.440, 218.143
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.580 - 2.690
R-factor	0.205
Rwork	0.204
R-free	0.23500
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hci
RMSD bond length	0.010
RMSD bond angle	1.060
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.700	2.770
High resolution limit [Å]	2.690	2.690
Rmerge	0.045	0.858
Number of reflections	38093	2640
<I/σ(I)>	20	2.8
Completeness [%]	99.4	95.2
Redundancy	6.7	6.3
CC(1/2)	0.999	0.915

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	295	100 mM HEPES (pH 7.0), 16% w/v Jeffamine ED-2003