7A8R

Structure of RecQL from Bos taurus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL18U1
Synchrotron site	SSRF
Beamline	BL18U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.978520
Spacegroup name	P 21 21 21
Unit cell lengths	55.576, 117.224, 203.819
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.160 - 2.310
R-factor	0.204
Rwork	0.201
R-free	0.25280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4u7d
RMSD bond length	0.004
RMSD bond angle	0.810
Data reduction software	XDS (BUILT 20190315))
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.18.2_3874)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.160	2.539
High resolution limit [Å]	2.310	2.320
Rmerge	0.083	0.733
Rmeas	0.090	0.866
Rpim	0.036	0.545
Number of reflections	44709	2235
<I/σ(I)>	15.2	1.5
Completeness [%]	91.5
Redundancy	5.9
CC(1/2)	0.999	0.443

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	PEG 20K 11-16% Hepes 100mM