7A8N

rsGreen0.7-K206A-N205L in the green-on state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	H 3 2
Unit cell lengths	140.728, 140.728, 73.246
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.845 - 2.100
R-factor	0.2109
Rwork	0.208
R-free	0.25750
RMSD bond length	0.002
RMSD bond angle	0.524
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHENIX (1.11)
Refinement software	PHENIX (1.11)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.845	46.840	2.160
High resolution limit [Å]	2.100	8.910	2.100
Rmerge	0.057	0.026	0.713
Rmeas	0.058	0.026	0.731
Rpim	0.013	0.007	0.162
Number of reflections	16005	239	1307
<I/σ(I)>	33.1
Completeness [%]	98.3	99.3	97.5
Redundancy	20.3	17.1	20.3
CC(1/2)	1.000	1.000	0.964

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM HEPES pH 7.5 25 % PEG 8000