7A8J

rsGreen0.7-K206A-H148V in the green-on state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-18
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	H 3 2
Unit cell lengths	140.616, 140.616, 73.004
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.592 - 1.850
R-factor	0.1912
Rwork	0.189
R-free	0.23150
RMSD bond length	0.009
RMSD bond angle	0.956
Data reduction software	XDS
Data scaling software	Aimless (0.5.23)
Phasing software	PHENIX (1.11)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.760	46.760	1.890
High resolution limit [Å]	1.850	9.060	1.850
Rmerge	0.051	0.018	0.664
Rmeas	0.053	0.020	0.699
Rpim	0.017	0.006	0.217
Total number of observations	241673
Number of reflections	23673	224	1443
<I/σ(I)>	28.4
Completeness [%]	100.0	99.1	100
Redundancy	10.2	9.9	10.3
CC(1/2)	1.000	1.000	0.932

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM MES pH 6.5 25 % PEG 550 mme 10 mM ZnSO4