7A8E

rsGreen0.7-K206A-F165W partially in the green-off state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-09
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97857
Spacegroup name	H 3 2
Unit cell lengths	140.744, 140.744, 72.897
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.756 - 2.200
R-factor	0.2078
Rwork	0.205
R-free	0.26130
RMSD bond length	0.002
RMSD bond angle	0.542
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHENIX (1.11)
Refinement software	PHENIX (1.11)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.760	46.760	2.270
High resolution limit [Å]	2.200	9.070	2.200
Rmerge	0.058	0.030	0.657
Rmeas	0.060	0.031	0.675
Rpim	0.013	0.007	0.154
Total number of observations	284967	3871	23391
Number of reflections	14148	225	1211
<I/σ(I)>	32.1	86.1	5.3
Completeness [%]	100.0	99.4	99.9
Redundancy	20.1	17.2	19.3
CC(1/2)	1.000	0.999	0.964

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM HEPES pH 7.5 25 % PEG 4000