7A88

rsGreen0.7-K206A-F145M in the green-off state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-07-09
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97857
Spacegroup name	H 3 2
Unit cell lengths	139.977, 139.977, 72.645
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.540 - 1.950
R-factor	0.179
Rwork	0.176
R-free	0.22610
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.922
Data reduction software	XDS
Data scaling software	Aimless (0.5.15)
Phasing software	PHASER (2.6.0)
Refinement software	PHENIX (1.11)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	46.540	46.540	2.000
High resolution limit [Å]	1.950	8.940	1.950
Rmerge	0.065	0.029	0.687
Rmeas	0.067	0.030	0.709
Rpim	0.016	0.008	0.174
Total number of observations	332432	3520	23052
Number of reflections	19962	235	1407
<I/σ(I)>	26.3	72.4	4.7
Completeness [%]	99.9	99.4	99.7
Redundancy	16.7	15	16.4
CC(1/2)	1.000	1.000	0.938

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM MMT 25 % PEG 1500