7A86
rsGreen0.7-K206A-F145L partially in the green-off state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-28 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.102, 58.290, 58.466 |
| Unit cell angles | 64.06, 71.06, 72.61 |
Refinement procedure
| Resolution | 44.717 - 1.900 |
| R-factor | 0.1826 |
| Rwork | 0.181 |
| R-free | 0.21050 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.913 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER (2.7.16) |
| Refinement software | PHENIX (1.11.1_2575) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 44.720 | 44.720 | 1.940 |
| High resolution limit [Å] | 1.900 | 9.110 | 1.900 |
| Rmerge | 0.035 | 0.019 | 0.490 |
| Rmeas | 0.042 | 0.023 | 0.575 |
| Rpim | 0.022 | 0.012 | 0.298 |
| Total number of observations | 140799 | 1254 | 9580 |
| Number of reflections | 40412 | 371 | 2624 |
| <I/σ(I)> | 14.8 | 44.1 | 2.5 |
| Completeness [%] | 96.6 | 96.4 | 96.2 |
| Redundancy | 3.5 | 3.4 | 3.7 |
| CC(1/2) | 0.999 | 0.999 | 0.903 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 100 mM MES pH 6.5 12 % PEG 20 000 4 % Pentaerythritol ethoxylate |
