7A86

rsGreen0.7-K206A-F145L partially in the green-off state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	P 1
Unit cell lengths	48.102, 58.290, 58.466
Unit cell angles	64.06, 71.06, 72.61

Refinement procedure

Resolution	44.717 - 1.900
R-factor	0.1826
Rwork	0.181
R-free	0.21050
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.913
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER (2.7.16)
Refinement software	PHENIX (1.11.1_2575)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	44.720	44.720	1.940
High resolution limit [Å]	1.900	9.110	1.900
Rmerge	0.035	0.019	0.490
Rmeas	0.042	0.023	0.575
Rpim	0.022	0.012	0.298
Total number of observations	140799	1254	9580
Number of reflections	40412	371	2624
<I/σ(I)>	14.8	44.1	2.5
Completeness [%]	96.6	96.4	96.2
Redundancy	3.5	3.4	3.7
CC(1/2)	0.999	0.999	0.903

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM MES pH 6.5 12 % PEG 20 000 4 % Pentaerythritol ethoxylate