7A85

rsGreen0.7-K206A-F145L in the green-on state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-02-18
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	I 1 2 1
Unit cell lengths	46.303, 60.910, 92.693
Unit cell angles	90.00, 96.85, 90.00

Refinement procedure

Resolution	43.241 - 1.520
R-factor	0.2129
Rwork	0.212
R-free	0.23680
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.879
Data reduction software	XDS
Data scaling software	Aimless (0.5.23)
Phasing software	PHASER (2.7.16)
Refinement software	PHENIX (1.11)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	43.240	43.240	1.550
High resolution limit [Å]	1.520	8.330	1.520
Rmerge	0.051	0.031	0.606
Rmeas	0.059	0.036	0.712
Rpim	0.030	0.017	0.367
Total number of observations	146055	1023	7044
Number of reflections	39087	259	1909
<I/σ(I)>	12.4	34.8	1.7
Completeness [%]	99.2	98.7	99.4
Redundancy	3.7	3.9	3.7
CC(1/2)	0.999	0.998	0.803

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM MES pH 6.5 12 % PEG 20 000 10 mM NaBr