7A83

rsGreen0.7-K206A-F145H in the green-on-state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	I 1 2 1
Unit cell lengths	45.750, 61.135, 91.831
Unit cell angles	90.00, 97.16, 90.00

Refinement procedure

Resolution	42.817 - 1.730
R-factor	0.1657
Rwork	0.164
R-free	0.19240
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.937
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHASER (2.7.16)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.820	42.820	1.760
High resolution limit [Å]	1.730	8.990	1.730
Rmerge	0.044	0.030	0.682
Rmeas	0.048	0.032	0.736
Rpim	0.018	0.013	0.275
Total number of observations	178548	1190	10045
Number of reflections	25749	201	1406
<I/σ(I)>	21	49.1	2.8
Completeness [%]	98.1	97.6	97
Redundancy	6.9	5.9	7.1
CC(1/2)	0.999	0.999	0.848

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	125 mM MES pH 6.0 9 % PEG 4000