7A82
rsGreen0.7-K206A-F145A partially in the green-off state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-28 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9801 |
Spacegroup name | H 3 2 |
Unit cell lengths | 141.363, 141.363, 73.108 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 40.808 - 1.900 |
R-factor | 0.2016 |
Rwork | 0.199 |
R-free | 0.24990 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.900 |
Data reduction software | XDS |
Data scaling software | Aimless (0.5.32) |
Phasing software | PHENIX (1.11) |
Refinement software | PHENIX (1.11) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.930 | 46.930 | 1.940 |
High resolution limit [Å] | 1.900 | 9.110 | 1.900 |
Rmerge | 0.045 | 0.034 | 0.774 |
Rmeas | 0.046 | 0.035 | 0.793 |
Rpim | 0.010 | 0.009 | 0.176 |
Total number of observations | 445440 | 3243 | 29009 |
Number of reflections | 22123 | 223 | 1410 |
<I/σ(I)> | 37.7 | 92.3 | 4.5 |
Completeness [%] | 100.0 | 99.1 | 100 |
Redundancy | 20.1 | 14.5 | 20.6 |
CC(1/2) | 1.000 | 0.999 | 0.967 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 200 mM NaCl 100 mM MES pH 6.0 20 % PEG 6000 200 mM NDSB-211 |