7A80
rsGreen0.7-K206A-E222V in the green-on state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-07-09 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.97857 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 69.822, 61.800, 112.121 |
| Unit cell angles | 90.00, 91.01, 90.00 |
Refinement procedure
| Resolution | 46.274 - 2.050 |
| R-factor | 0.1854 |
| Rwork | 0.183 |
| R-free | 0.23430 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.557 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.32) |
| Phasing software | PHASER (2.7.16) |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.270 | 46.270 | 2.100 |
| High resolution limit [Å] | 2.050 | 9.170 | 2.050 |
| Rmerge | 0.097 | 0.049 | 0.682 |
| Rmeas | 0.105 | 0.054 | 0.739 |
| Rpim | 0.041 | 0.022 | 0.282 |
| Total number of observations | 405037 | 3998 | 30415 |
| Number of reflections | 60229 | 715 | 4424 |
| <I/σ(I)> | 13.2 | 28 | 2.9 |
| Completeness [%] | 99.9 | 98.3 | 100 |
| Redundancy | 6.7 | 5.6 | 6.9 |
| CC(1/2) | 0.998 | 0.997 | 0.861 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 26 % PEG 4000 500 mM Mg(NO3) 50 mM HEPES pH 7.5 |
