7A7X

rsGreen0.7-F145M in the green-off state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-06-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	P 21 21 21
Unit cell lengths	31.916, 62.084, 106.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.408 - 1.850
R-factor	0.1503
Rwork	0.147
R-free	0.20700
RMSD bond length	0.006
RMSD bond angle	0.877
Data reduction software	XDS
Data scaling software	Aimless (0.6.2)
Phasing software	PHENIX (1.11)
Refinement software	PHENIX (1.11)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.410	40.410	1.890
High resolution limit [Å]	1.850	9.060	1.850
Rmerge	0.084	0.041	0.644
Rmeas	0.088	0.044	0.672
Rpim	0.025	0.014	0.191
Number of reflections	18843	208	1160
<I/σ(I)>	19.1
Completeness [%]	100.0	99.2	100
Redundancy	12.5	9.2	12.6
CC(1/2)	0.999	0.999	0.934

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM MMT pH 8.0 25 % PEG 1500