7A7R

rsGreenF-K206A in the green-off state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 2
Synchrotron site	SOLEIL
Beamline	PROXIMA 2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-06-28
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.9801
Spacegroup name	P 41 21 2
Unit cell lengths	106.185, 106.185, 35.907
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.487 - 2.350
R-factor	0.1774
Rwork	0.174
R-free	0.24320
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.001
RMSD bond angle	0.459
Data reduction software	XDS
Data scaling software	Aimless (0.5.32)
Phasing software	PHENIX (1.11)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.490	47.490	2.430
High resolution limit [Å]	2.350	9.100	2.350
Rmerge	0.146	0.062	0.667
Rmeas	0.152	0.067	0.695
Rpim	0.043	0.022	0.196
Total number of observations		1847	11045
Number of reflections	9057	199	870
<I/σ(I)>	16.3	28.2	4.7
Completeness [%]	100.0	99.5	100
Redundancy	12.4	9.3	12.7
CC(1/2)	0.997	0.995	0.915

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	200 mM NaCl 100 mM MES pH 6.0 20 % PEG 6000