7A7K

rsEGFP in the green-on state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-09-11
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.8856
Spacegroup name	P 1 21 1
Unit cell lengths	47.477, 51.400, 47.622
Unit cell angles	90.00, 97.76, 90.00

Refinement procedure

Resolution	47.186 - 1.550
R-factor	0.1706
Rwork	0.169
R-free	0.21080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4eul
RMSD bond length	0.006
RMSD bond angle	0.883
Data reduction software	XDS
Data scaling software	Aimless (0.2.7)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.11)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.190	47.190	1.580
High resolution limit [Å]	1.550	8.490	1.550
Rmerge	0.067	0.048	0.751
Rmeas	0.079	0.056	0.876
Rpim	0.040	0.028	0.444
Total number of observations	122879
Number of reflections	33023	223	1610
<I/σ(I)>	10.4
Completeness [%]	99.7	99.2	99.9
Redundancy	3.7	3.4	3.8
CC(1/2)	0.997	0.996	0.558

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	200 mM (NH4)SO4 100 mM Bis-trip pH 5.5 25 % PEG 3350