7A7K
rsEGFP in the green-on state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-09-11 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.8856 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.477, 51.400, 47.622 |
| Unit cell angles | 90.00, 97.76, 90.00 |
Refinement procedure
| Resolution | 47.186 - 1.550 |
| R-factor | 0.1706 |
| Rwork | 0.169 |
| R-free | 0.21080 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4eul |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.883 |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.2.7) |
| Phasing software | PHASER (2.5.6) |
| Refinement software | PHENIX (1.11) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 47.190 | 47.190 | 1.580 |
| High resolution limit [Å] | 1.550 | 8.490 | 1.550 |
| Rmerge | 0.067 | 0.048 | 0.751 |
| Rmeas | 0.079 | 0.056 | 0.876 |
| Rpim | 0.040 | 0.028 | 0.444 |
| Total number of observations | 122879 | ||
| Number of reflections | 33023 | 223 | 1610 |
| <I/σ(I)> | 10.4 | ||
| Completeness [%] | 99.7 | 99.2 | 99.9 |
| Redundancy | 3.7 | 3.4 | 3.8 |
| CC(1/2) | 0.997 | 0.996 | 0.558 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 200 mM (NH4)SO4 100 mM Bis-trip pH 5.5 25 % PEG 3350 |
