7A71

Structure of G132S BlaC from Mycobacterium tuberculosis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.000
Spacegroup name	P 21 21 21
Unit cell lengths	53.296, 54.316, 78.852
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.770 - 1.400
R-factor	0.13153
Rwork	0.130
R-free	0.16083
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gdn
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.730	1.420
High resolution limit [Å]	1.400	1.400
Rpim	0.083	0.698
Number of reflections	45293	2169
<I/σ(I)>	8.3	2.6
Completeness [%]	98.9
Redundancy	3.7	3.3
CC(1/2)	0.989	0.654

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	293	0.1 M Sodium Cacodylate, 0.2 M Sodium Chloride, 2 M Ammonium Sulfate