7A6Y
Structure of 14-3-3 gamma in complex with DAPK2 peptide stabilized by FC-A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-13 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 1.54187 |
| Spacegroup name | H 3 |
| Unit cell lengths | 205.502, 205.502, 74.274 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 29.680 - 2.500 |
| R-factor | 0.2248 |
| Rwork | 0.223 |
| R-free | 0.25890 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b05 |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.537 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.680 | 2.589 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.036 | 0.219 |
| Rmeas | 0.041 | 0.292 |
| Number of reflections | 40349 | 4042 |
| <I/σ(I)> | 26.4 | 3.6 |
| Completeness [%] | 99.6 | 99.65 |
| Redundancy | 4.23 | |
| CC(1/2) | 0.999 | 0.916 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290.15 | HEPES, MgCl2, PEG400, hexafluoro-2-propanol, FC-A |
