7A6R
Structure of 14-3-3 gamma in complex with DAPK2 peptide containing the 14-3-3 binding motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-13 |
| Detector | DECTRIS PILATUS 300K |
| Wavelength(s) | 1.54187 |
| Spacegroup name | H 3 |
| Unit cell lengths | 205.620, 205.620, 74.091 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.640 - 2.700 |
| R-factor | 0.2386 |
| Rwork | 0.237 |
| R-free | 0.27540 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2b05 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.404 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.640 | 2.797 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.084 | 0.435 |
| Rmeas | 0.096 | 0.539 |
| Number of reflections | 31982 | 3165 |
| <I/σ(I)> | 13.5 | 2.4 |
| Completeness [%] | 99.7 | |
| Redundancy | 4.39 | |
| CC(1/2) | 0.997 | 0.846 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 290.15 | HEPES, MgCl2, PEG400, hexafluoro-2-propanol |
