7A6R

Structure of 14-3-3 gamma in complex with DAPK2 peptide containing the 14-3-3 binding motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-03-13
Detector	DECTRIS PILATUS 300K
Wavelength(s)	1.54187
Spacegroup name	H 3
Unit cell lengths	205.620, 205.620, 74.091
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	30.640 - 2.700
R-factor	0.2386
Rwork	0.237
R-free	0.27540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2b05
RMSD bond length	0.002
RMSD bond angle	0.404
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.640	2.797
High resolution limit [Å]	2.700	2.700
Rmerge	0.084	0.435
Rmeas	0.096	0.539
Number of reflections	31982	3165
<I/σ(I)>	13.5	2.4
Completeness [%]	99.7
Redundancy	4.39
CC(1/2)	0.997	0.846

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	290.15	HEPES, MgCl2, PEG400, hexafluoro-2-propanol