7A57

La Crosse Virus Envelope Glycoprotein Gc W1066H Mutant Fusion Domains in Postfusion Conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-02
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9786
Spacegroup name	P 61 2 2
Unit cell lengths	170.873, 170.873, 379.220
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	48.057 - 3.155
R-factor	0.2009
Rwork	0.199
R-free	0.22630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7a56
RMSD bond length	0.005
RMSD bond angle	0.924
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHENIX
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.173	3.485
High resolution limit [Å]	3.155	3.155
Rmerge	0.132	1.076
Rmeas	0.144	1.177
Rpim	0.057	0.470
Number of reflections	38252	1913
<I/σ(I)>	9.2	1.8
Completeness [%]	67.1	13.3
Redundancy	6.3	6
CC(1/2)	0.998	0.717

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291.15	0.2 uL of 8.5 mg/mL trimeric LACV Gc(918-1364) W1066H in 20 mM Tris-Cl pH 8.0, 150 mM NaCl were added to 0.2 uL of reservoir solution containing 0.1 M Bis-Tris propane pH 7 and 1.2 M K/Na tartrate (final pH: 8.0)