7A50
Crystal structure of the APH coiled-coil in complex with nanobody Nb26
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-07-22 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.978570 |
Spacegroup name | H 3 2 |
Unit cell lengths | 198.220, 198.220, 58.190 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.320 - 1.999 |
R-factor | 0.1843 |
Rwork | 0.182 |
R-free | 0.22570 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Modeler |
RMSD bond length | 0.009 |
RMSD bond angle | 1.001 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.320 | 2.071 |
High resolution limit [Å] | 1.999 | 1.999 |
Rmeas | 0.222 | |
Number of reflections | 29399 | 2821 |
<I/σ(I)> | 8.07 | 1.39 |
Completeness [%] | 99.5 | 95.85 |
Redundancy | 2.07 | 3.22 |
CC(1/2) | 0.991 | 0.718 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5 | 298 | 0.2 M calcium chloride, 0.1 M sodium acetate pH 5, 20 % w/v PEG 6000 |