7A4U
Crystal structure of lid-truncated apo BiP in an oligomeric state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-30 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9760 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 50.333, 51.289, 119.716 |
Unit cell angles | 90.00, 100.17, 90.00 |
Refinement procedure
Resolution | 58.910 - 1.770 |
R-factor | 0.1864 |
Rwork | 0.185 |
R-free | 0.21700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6hab |
RMSD bond length | 0.006 |
RMSD bond angle | 1.341 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | REFMAC (5.8.0266) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.920 | 1.810 |
High resolution limit [Å] | 1.770 | 1.770 |
Number of reflections | 58872 | 3347 |
<I/σ(I)> | 9.9 | 1.1 |
Completeness [%] | 99.9 | 99.8 |
Redundancy | 3.2 | |
CC(1/2) | 0.975 | 0.531 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | 0.1 M BIS-TRIS propane pH 7.5, 0.2 M trisodium citrate dihydrate, 20% PEG 3350 |