7A40
Nucleotide-free OSM-3 kinesin motor domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.979338 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 42.770, 155.180, 63.720 |
| Unit cell angles | 90.00, 108.97, 90.00 |
Refinement procedure
| Resolution | 47.590 - 2.297 |
| R-factor | 0.2265 |
| Rwork | 0.225 |
| R-free | 0.25390 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7a3z |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.020 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3 (6-FEB-2020)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.600 | 2.400 |
| High resolution limit [Å] | 2.297 | 2.300 |
| Rmeas | 0.121 | |
| Number of reflections | 34809 | 5500 |
| <I/σ(I)> | 8.6 | |
| Completeness [%] | 99.6 | 95.4 |
| Redundancy | 7 | 6.6 |
| CC(1/2) | 0.997 | 0.625 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | 20% (W/V) polyethylene glycol 3350, 170 mM (NH4)2SO4, and 0.1 M Mes buffer at pH 6.5 |
