7A3T
Crystal structure of dengue 3 virus envelope glycoprotein in complex with the Fab fragment of the broadly neutralizing human antibody EDE1 C8
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-06 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 1.0 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 110.309, 110.309, 217.337 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 29.530 - 2.650 |
R-factor | 0.2096 |
Rwork | 0.207 |
R-free | 0.25400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | D_1292109938 and 4UTA |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX (1.17.1_3660) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.150 | 2.750 |
High resolution limit [Å] | 2.650 | 2.650 |
Rmerge | 0.320 | |
Number of reflections | 44949 | 4497 |
<I/σ(I)> | 5.1 | 0.4 |
Completeness [%] | 99.5 | 95.8 |
Redundancy | 11.5 | |
CC(1/2) | 0.990 | 0.095 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 100 mM Na3 Citrate pH 5.6, 15% w/v PEG 4,000, 200 mM (NH4)2 SO4 |