7A3R

Crystal structure of dengue 1 virus envelope glycoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2010-07-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	105.190, 105.190, 180.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.850 - 3.600
R-factor	0.2534
Rwork	0.250
R-free	0.29540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1uzg
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.100	3.700
High resolution limit [Å]	3.500	3.500
Rmerge	0.219	0.476
Number of reflections	13279	1797
<I/σ(I)>	9.9
Completeness [%]	98.7	94.7
Redundancy	6.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		291	50 mM Tris pH 8.0, 18% PEG 3,350, 400 mM Mg Acetate, 200 mM NaCl