7A3F
Crystal structure of apo DPP9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-08-28 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 95.170, 95.310, 127.196 |
| Unit cell angles | 86.56, 102.54, 102.44 |
Refinement procedure
| Resolution | 46.890 - 2.900 |
| R-factor | 0.2264 |
| Rwork | 0.223 |
| R-free | 0.29000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6eor |
| RMSD bond length | 0.003 |
| RMSD bond angle | 1.272 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.890 | 46.890 | 2.970 |
| High resolution limit [Å] | 2.900 | 26.100 | 2.900 |
| Rmerge | 0.087 | 0.020 | 0.491 |
| Rmeas | 0.115 | 0.026 | 0.645 |
| Total number of observations | 207713 | ||
| Number of reflections | 92941 | 107 | 6869 |
| <I/σ(I)> | 7.24 | 22.3 | 2.06 |
| Completeness [%] | 98.4 | 82.3 | 98.1 |
| Redundancy | 2.235 | 2.112 | 2.299 |
| CC(1/2) | 0.991 | 0.999 | 0.806 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.08 M Na-Cacodilate pH 5.25, 0.16 M Ca-Acetate, 30 % Glycerol, 10 % PEG 8K |
