7A1R
Crystal structure of the C2B domain of Trypanosoma brucei extended synaptotagmin (E-Syt)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 77 |
Detector technology | PIXEL |
Collection date | 2015-09-07 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.873 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 54.908, 57.532, 84.570 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.860 - 1.500 |
R-factor | 0.1705 |
Rwork | 0.170 |
R-free | 0.19070 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | AutoSol |
Refinement software | PHENIX (1.18_3845) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.550 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.083 | 0.978 |
Rmeas | 0.092 | 1.035 |
Rpim | 0.039 | 0.429 |
Number of reflections | 43547 | 4297 |
<I/σ(I)> | 10.39 | 1.37 |
Completeness [%] | 99.6 | 99.8 |
Redundancy | 5.4 | 5.5 |
CC(1/2) | 0.998 | 0.644 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 295 | 0.1 M sodium cacodylate (pH 6.5), 0.15 M sodium acetate, 28% (v/v) polyethylene glycol 8,000 |