7A1P

FACTOR INHIBITING HIF-1 ALPHA IN COMPLEX WITH ZN(II), 4-propyl-2-oxoglutarate, AND CONSENSUS ANKYRIN REPEAT DOMAIN (20-MER)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-30
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97950
Spacegroup name	P 41 21 2
Unit cell lengths	85.945, 85.945, 147.628
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.200 - 1.760
R-factor	0.1623
Rwork	0.161
R-free	0.19110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h2k
RMSD bond length	0.010
RMSD bond angle	1.017
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.275	1.790
High resolution limit [Å]	1.759	1.759
Rmerge	0.076	6.504
Number of reflections	104634	2745
<I/σ(I)>	22.5	0.6
Completeness [%]	100.0	100
Redundancy	26.6	28
CC(1/2)	1.000	0.365

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	1.6M AMMONIUM SULPHATE, 6% PEG 400, 0.1M HEPES PH 7.5