7A1M

FACTOR INHIBITING HIF-1 ALPHA IN COMPLEX WITH ZN(II) AND 3-propyl-2-oxoglutarate

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-30
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.97950
Spacegroup name	P 41 21 2
Unit cell lengths	85.954, 85.954, 148.957
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	56.290 - 2.180
R-factor	0.2216
Rwork	0.220
R-free	0.24530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1h2k
RMSD bond length	0.003
RMSD bond angle	0.585
Data reduction software	XDS
Data scaling software	autoPROC
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.448	2.216
High resolution limit [Å]	2.178	2.178
Rmerge	0.156	6.807
Number of reflections	51777	1487
<I/σ(I)>	17.1	0.5
Completeness [%]	93.7	100
Redundancy	25.8	24.2
CC(1/2)	1.000	0.414

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	1.6M AMMONIUM SULPHATE, 6% PEG 400, 0.1M HEPES PH 7.5