7A18

50S Deinococcus radiodurans ribosome bounded with mycinamicin IV

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE MASSIF-3
Synchrotron site	ESRF
Beamline	MASSIF-3
Temperature [K]	80
Detector technology	PIXEL
Collection date	2017-05-11
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.968
Spacegroup name	I 2 2 2
Unit cell lengths	169.590, 410.128, 690.477
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 3.400
R-factor	0.2854
Rwork	0.284
R-free	0.31820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3pio
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX (1.12_2829)
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.074	50.000	3.360
High resolution limit [Å]	3.300	8.940	3.300
Rmerge	0.295	0.202	0.920
Rmeas	0.323	0.220	1.049
Rpim	0.128	0.084	0.483
Number of reflections	344576	17791	16717
<I/σ(I)>	7.3
Completeness [%]	97.3	96.6	95.3
Redundancy	5	6.3	3.8
CC(1/2)		0.833	0.443

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.8	293.15	Ribosome solution containing 0.0065 mM (180 A/ml) of D50S in 10 mM Hepes pH=7.8, 15 mM MgCl2 and 75 mM NH4Cl crystallization buffer was mixed with 10 mM spermidine, 1 % ethanol and 0.5 % 2-ethyl-1,3-hexanediol precipitants. A 0.005 ml crystallization drop was hanged over 10 % ethanol and 5% 2-ethyl-1,3-hexanediol in ddw reservoir.