7A0V

Crystal structure of the 5-phosphatase domain of Synaptojanin1 in complex with a nanobody

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-27
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.980105
Spacegroup name	C 1 2 1
Unit cell lengths	168.874, 108.793, 100.974
Unit cell angles	90.00, 120.72, 90.00

Refinement procedure

Resolution	86.810 - 2.300
R-factor	0.1992
Rwork	0.196
R-free	0.25230
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1i9y 3n9v 3mtc 4cmn 4nc2
RMSD bond length	0.002
RMSD bond angle	0.480
Data reduction software	XDS (Mar 15, 2019 (BUILT 20190806))
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER (2.6.0)
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	86.810	2.430
High resolution limit [Å]	2.297	2.300
Rmerge	0.133
Rmeas	0.143
Rpim	0.054
Number of reflections	53823	1511
<I/σ(I)>	11.2
Completeness [%]	92.3
Redundancy	7
CC(1/2)	0.997	0.512

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293	15% PEG 4000, 0.1 M sodium citrate pH 5, 10% 2-propanol