7A0N

Structure of TSC1 NTD and linker domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-28
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9802
Spacegroup name	P 21 21 21
Unit cell lengths	97.540, 160.790, 255.260
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.993 - 4.300
R-factor	0.2796
Rwork	0.278
R-free	0.30610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7a0m
RMSD bond length	0.004
RMSD bond angle	1.028
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	4.530
High resolution limit [Å]	4.300	4.300
Rmeas	0.367	2.285
Number of reflections	27707	3968
<I/σ(I)>	6.7	1.9
Completeness [%]	98.7
Redundancy	15.5
CC(1/2)	0.994	0.917

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1 M HEPES pH 7.3, 30 % glycerol, 7 % 2-propanol, 0.01 M phenol