7A0M

TSC1 N-terminal domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-03-17
Detector	RAYONIX MX-225
Wavelength(s)	0.977
Spacegroup name	C 1 2 1
Unit cell lengths	141.085, 150.744, 111.880
Unit cell angles	90.00, 108.47, 90.00

Refinement procedure

Resolution	44.900 - 2.320
R-factor	0.2408
Rwork	0.240
R-free	0.26990
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.596
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX ((1.12rc1_2807: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.900	2.400
High resolution limit [Å]	2.320	2.320
Rmeas	0.064	0.696
Number of reflections	95121	15143
<I/σ(I)>	13.2	2.2
Completeness [%]	98.9
Redundancy	3.4
CC(1/2)	0.998	0.764

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	0.1 M bicine trizma (pH 8.5), 16% (w/v) PEG 3350, 12.5% (w/v) PEG 1000, 12.5 % (v/v) MPD, 0.03 M sodium nitrate, 0.03 M sodium phosphate dibasic and 0.03 M ammonium sulfate