7A0K

Crystal structure of the entire ectodomain from the Physcomitrella patens receptor kinase CR4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-11
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.999894
Spacegroup name	P 1 21 1
Unit cell lengths	88.579, 183.962, 98.173
Unit cell angles	90.00, 96.05, 90.00

Refinement procedure

Resolution	45.870 - 2.700
R-factor	0.2174
Rwork	0.216
R-free	0.24650
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7a0j
RMSD bond length	0.003
RMSD bond angle	0.594
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.17.1_3660: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.870	2.860
High resolution limit [Å]	2.700	2.700
Rmeas	0.151	1.890
Number of reflections	85660	13775
<I/σ(I)>	12	1
Completeness [%]	99.9	99.7
Redundancy	7	6.6
CC(1/2)	0.998	0.464

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	15 % PEG 4,000, 0.2 M imidazole malate pH 7.0