6ZZZ

Crystal structure of yeast Sec62 cytoplasmic domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM02
Synchrotron site	ESRF
Beamline	BM02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-19
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97958
Spacegroup name	P 65 2 2
Unit cell lengths	99.206, 99.206, 155.657
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.260 - 2.540
R-factor	0.2225
Rwork	0.221
R-free	0.25980
Structure solution method	SAD
RMSD bond length	0.013
RMSD bond angle	1.729
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.260	2.630
High resolution limit [Å]	2.540	2.540
Rmerge	0.107	1.869
Rmeas	0.109	1.894
Rpim	0.018
Number of reflections	15563	1495
<I/σ(I)>	26.56	2.03
Completeness [%]	99.8
Redundancy	38.1
CC(1/2)	1.000	0.949

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	100mM MES pH 5.7-5.9, 2 M (NH4)2SO4