6ZZJ
Crystal structure of the catalytic domain of Corynebacterium glutamicum acetyltransferase AceF (E2p) in complex with oxidized CoA.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 1 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-07-20 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.980110 |
Spacegroup name | P 63 2 2 |
Unit cell lengths | 73.739, 73.739, 192.186 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.050 - 1.350 |
R-factor | 0.1691 |
Rwork | 0.168 |
R-free | 0.19410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6zzi |
RMSD bond length | 0.010 |
RMSD bond angle | 1.040 |
Data reduction software | autoPROC |
Data scaling software | autoPROC (1.0.5) |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.050 | 1.370 |
High resolution limit [Å] | 1.350 | 1.350 |
Rpim | 0.020 | 0.545 |
Number of reflections | 68504 | 3111 |
<I/σ(I)> | 16.1 | 1.1 |
Completeness [%] | 99.6 | 92.8 |
Redundancy | 25 | 21.2 |
CC(1/2) | 0.998 | 0.844 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | 0.1 M Hepes-Na pH 7.5, 1.56 M tri-sodium citrate |