6ZZI
Crystal structure of the catalyic domain of Corynebacterium glutamicum acetyltransferase AceF (E2p).
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9801 |
| Spacegroup name | P 3 |
| Unit cell lengths | 158.278, 158.278, 63.016 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 79.140 - 1.932 |
| R-factor | 0.1672 |
| Rwork | 0.166 |
| R-free | 0.18510 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4n72 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.910 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 79.139 | 1.966 |
| High resolution limit [Å] | 1.932 | 1.932 |
| Rpim | 0.043 | 0.369 |
| Number of reflections | 131357 | 6564 |
| <I/σ(I)> | 14 | |
| Completeness [%] | 99.3 | |
| Redundancy | 3.8 | |
| CC(1/2) | 0.998 | 0.736 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.1 M Hepes-Na pH 7.5, 5% PEG4000, 30% MPD |
