6ZZG
MB_CRS6-1 bound to CrSAS-6_N
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-23 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 164.451, 347.187, 94.851 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.400 - 2.930 |
| R-factor | 0.2228 |
| Rwork | 0.221 |
| R-free | 0.25380 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q0y |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.782 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.15_3459) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.400 | 3.070 |
| High resolution limit [Å] | 2.930 | 2.930 |
| Number of reflections | 273558 | 9570 |
| <I/σ(I)> | 5.98 | |
| Completeness [%] | 99.5 | |
| Redundancy | 4.5 | |
| CC(1/2) | 0.998 | 0.517 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 0.3 M Sodium acetate trihydrate, 10%w/vPEG8000, 10%w/v PEG 1000, 4% v/v 1,1,1,3,3,3-Hexafluoro-2-propanol, 0.1 M Tris pH 8.5 |
