6ZZD
MB_CRS6-13 bound to CrSAS-6_N
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-03-18 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 107.888, 65.722, 90.423 |
| Unit cell angles | 90.00, 109.03, 90.00 |
Refinement procedure
| Resolution | 43.060 - 2.050 |
| R-factor | 0.2002 |
| Rwork | 0.199 |
| R-free | 0.22810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q0y |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.171 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15_3459) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.060 | 2.125 |
| High resolution limit [Å] | 2.050 | 2.051 |
| Rmerge | 0.086 | 1.451 |
| Number of reflections | 255461 | 3640 |
| <I/σ(I)> | 13.03 | |
| Completeness [%] | 98.7 | |
| Redundancy | 6.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 15% PEG 4000, 0.2M magnesium chloride hexahydrate, 0.1 M Tris pH 8.5 |
