6ZZA
Crystal structure of CbpB in complex with c-di-AMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-06-09 |
Detector | DECTRIS EIGER2 X 9M |
Wavelength(s) | 0.9801 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 93.830, 93.830, 33.600 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 31.050 - 2.491 |
R-factor | 0.189 |
Rwork | 0.186 |
R-free | 0.24500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6zz9 |
RMSD bond length | 0.014 |
RMSD bond angle | 1.750 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.050 | 2.580 |
High resolution limit [Å] | 2.491 | 2.491 |
Rmerge | 0.101 | 0.620 |
Rmeas | 0.112 | 0.689 |
Rpim | 0.048 | 0.291 |
Number of reflections | 6110 | 578 |
<I/σ(I)> | 9.79 | 2.03 |
Completeness [%] | 99.1 | 93.21 |
Redundancy | 5.3 | 5.1 |
CC(1/2) | 0.996 | 0.798 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 291 | 200 mM ammonium sulfate, 20% w/v PEG 4000 |