6ZZ8
MB_CRS6-15 bound to CrSAS-6_6HR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-21 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 257.160, 257.160, 125.289 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.630 - 3.730 |
| R-factor | 0.2272 |
| Rwork | 0.225 |
| R-free | 0.26710 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3q0x |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.713 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.15_3459) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.630 | 3.780 |
| High resolution limit [Å] | 3.730 | 3.730 |
| Rmerge | 0.044 | 0.637 |
| Number of reflections | 88072 | 8552 |
| <I/σ(I)> | 10.27 | |
| Completeness [%] | 99.0 | |
| Redundancy | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 40% MPD, 5% PEG 8000, 0.1M MES pH 6.5, 0.4M DL-Glutamic acid monohydrate |
