6ZYG
Emfourin (M4in) from Serratia proteamaculans, M4 family peptidase inhibitor
Spectrometer
Experimental method: SOLUTION NMR, SOLUTION SCATTERING
| Spectrometer ID | Spectrometer maker | Spectrometer model | Spectrometer type | Spectrometer field strength |
| 1 | Bruker | AVANCE | 600 |
Experiment
| experiment id | conditions id | solution id | Experiment type |
| 1 | 1 | 1 | 2D 1H-15N HSQC |
| 10 | 3 | 1 | 3D HNCA |
| 11 | 3 | 1 | 3D HN(CO)CA |
| 12 | 3 | 1 | 3D HNCACB |
| 13 | 3 | 1 | 3D HNHA |
| 14 | 3 | 1 | 3D HNHB |
| 15 | 3 | 1 | 3D HCCH-TOCSY |
| 16 | 3 | 1 | 3D 1H-15N TOCSY |
| 17 | 3 | 1 | 3D 1H-15N NOESY |
| 18 | 3 | 1 | 3D 1H-13C NOESY aliphatic |
| 19 | 3 | 1 | 3D 1H-13C NOESY aromatic |
| 2 | 2 | 1 | 2D 1H-15N HSQC |
| 20 | 3 | 1 | 2D 1H-15N TROSY-HSQC |
| 21 | 3 | 1 | 2D 1H-13C HSQC aliphatic, constant time |
| 22 | 3 | 1 | 2D 1H-13C HSQC aromatic, constant time |
| 23 | 3 | 1 | 2D 1H-13C{15N} spin-echo difference CT-HSQC, experiment to measure J(C-N) |
| 24 | 3 | 1 | 2D 1H-13C{13CO} spin-echo difference CT-HSQC, experiment to measure J(C-CO) |
| 25 | 3 | 1 | 2D 1H-13C{13CA} spin-echo difference CT-HSQC, experiment to measure J(CD-CA) |
| 3 | 3 | 1 | 2D 1H-15N HSQC |
| 4 | 4 | 1 | 2D 1H-15N HSQC |
| 5 | 5 | 1 | 2D 1H-15N HSQC |
| 6 | 3 | 1 | 2D 1H-13C HSQC aliphatic |
| 7 | 3 | 1 | 2D 1H-13C HSQC aromatic |
| 8 | 3 | 1 | 3D HNCO |
| 9 | 3 | 1 | 3D HN(CA)CO |
NMR Sample
| conditions id | NMR sample pH | NMR sample pressure | NMR sample temperature |
| 1 | 6.5 | 1 | 298 |
| 2 | 6.5 | 1 | 300 |
| 3 | 6.5 | 1 | 303 |
| 4 | 6.5 | 1 | 305 |
| 5 | 6.5 | 1 | 308 |
Conformers
| Conformers Calculated Total Number | 100 |
| Conformers Submitted Total Number | 20 |
Refinement Statistics
Experimental method: SOLUTION NMR, SOLUTION SCATTERING
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| Resolution limits | - | ||
