6ZYB

Sarcin-Ricin Loop RNA from Ecoli with a C2667-2'-OCF3 modification

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-24
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.00
Spacegroup name	P 43
Unit cell lengths	29.500, 29.500, 77.130
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.500 - 0.900
R-factor	0.1319
Rwork	0.131
R-free	0.14940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3dvz
RMSD bond length	0.007
RMSD bond angle	1.552
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	MOLREP
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.000	0.920
High resolution limit [Å]	0.900	0.900
Number of reflections	96381	3626
<I/σ(I)>	45
Completeness [%]	100.0
Redundancy	39.1
CC(1/2)	1.000	0.555

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	3.2 M ammonium sulfate, 50 mM potassium MOPS, pH 7.0, 10 mM magnesium chloride, 10 mM manganese chloride, VAPOR DIFFUSION, SITTING DROP, temperature 293K