6ZXM
Diguanylate cyclase DgcR in complex with c-di-GMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 93.15 |
| Detector technology | PIXEL |
| Collection date | 2017-02-24 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 122.603, 72.917, 125.729 |
| Unit cell angles | 90.00, 118.20, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.300 |
| R-factor | 0.228 |
| Rwork | 0.225 |
| R-free | 0.29900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v0n |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.988 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 3.400 |
| High resolution limit [Å] | 3.300 | 3.300 |
| Rmerge | 0.160 | 0.682 |
| Number of reflections | 28038 | 1877 |
| <I/σ(I)> | 6.75 | |
| Completeness [%] | 99.0 | 91.78 |
| Redundancy | 3.78 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291.15 | 0.2 M Potassium thiocyanate; 0.1 M Tris pH 7.5; 25% PEG 2000 MME |
