6ZX8
Racemic compound of RNA duplexes.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-02-02 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.283 |
| Spacegroup name | P 1 |
| Unit cell lengths | 21.018, 25.804, 38.414 |
| Unit cell angles | 104.93, 96.59, 92.11 |
Refinement procedure
| Resolution | 36.809 - 1.981 |
| Rwork | 0.172 |
| R-free | 0.25920 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6zq9 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.657 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 2.100 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmeas | 0.140 | 0.677 |
| Number of reflections | 8769 | 1215 |
| <I/σ(I)> | 4.31 | |
| Completeness [%] | 81.4 | 69.2 |
| Redundancy | 1.9 | |
| CC(1/2) | 0.985 | 0.782 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 293 | 0.2 M zinc acetate, 0.1 M cacodylate buffer, 18% polyethylene glycol 8000. |
