6ZX4
Neisseria gonorrhoeae transaldolase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2) |
| Synchrotron site | PETRA III, EMBL c/o DESY |
| Beamline | P14 (MX2) |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2019-12-13 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.7293 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 42.260, 82.790, 90.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.870 - 0.960 |
| R-factor | 0.1181 |
| Rwork | 0.117 |
| R-free | 0.13620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3clm |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.188 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX (1.13_2998) |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.870 | 0.990 |
| High resolution limit [Å] | 0.960 | 0.960 |
| Rmeas | 0.040 | 1.270 |
| Number of reflections | 190503 | 54087 |
| <I/σ(I)> | 16.4 | 1.1 |
| Completeness [%] | 98.5 | 97.9 |
| Redundancy | 3.4 | 3.27 |
| CC(1/2) | 0.990 | 0.530 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | PEG 8000, sodium chloride, citric acid, sodium phosphate, glycylglycine |
