6ZWX
Crystal structure of E. coli RNA helicase HrpA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-10-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.918 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.127, 116.544, 92.591 |
| Unit cell angles | 90.00, 98.61, 90.00 |
Refinement procedure
| Resolution | 49.160 - 2.700 |
| R-factor | 0.2235 |
| Rwork | 0.221 |
| R-free | 0.27630 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.617 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | AutoSol |
| Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.249 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Number of reflections | 23139 | 3427 |
| <I/σ(I)> | 12.9 | 0.8 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 12.1 | |
| CC(1/2) | 0.990 | 0.560 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.3 | 291 | 9 % [w/v] PEG 400, 0.2 M NaCl, 0.1 M Hepes |
